Movie S1: Beta cells distribution in pig pancreas by SHANEL histology
SHANEL histology revealed individual or groups of beta cells within islets of INS-EGFP transgenic pig pancreas that is several centimeters in size. 3D reconstruction of nanobody boosted EGFP transgenic pancreas imaged by light-sheet microscopy. After SHANEL, all labelled beta cells became evident in the islets throughout the pancreas. In the second part of the movie, segmented EGFP+ islets are shown.

Movie S2: 3D imaging of intact human eye using SHANEL technology
3D reconstruction of intact human eye imaged by light-sheet microscopy. SHANEL technology revealed structural details of sclera, iris and suspensory ligament in intact human eye.

Movie S3: Abeta plaques accumulations in 92 years large human brain slice by SHANEL histology
Upright confocal microscopy imaging of 0.4 cm thickness of a human brain slice with the cellular resolution (before clearing size: 10 x 7 x 1.5 cm; after clearing size 7.5 x 5 x 1.1 cm). The large scan shows the Abeta plaques are heavily accumulated in specific cortex regions including middle temporal gyrus (MTG) and cingulate gyrus (CG).

Movie S4: SHANEL histology on centimeters size human brain tissues for microglia labeling
3D reconstruction of Iba1 antibody labeled microglia cells were evident throughout the centimeters size human brain tissue imaged by light-sheet microscopy. Individual microglia across the gray matter and white matter showed ramified and unramified morphological differences.

Movie S5: SHANEL histology on centimeters size human brain tissues for neural process labeling
3D reconstruction of Tyrosine hydroxylase (TH) antibody labeled neuronal processes were evident throughout centimeters size human brain tissue imaged by light-sheet microscopy. Individual axonal extension across the gray matter and white matter were visible.

Movie S6: Cellular and molecular investigation of human kidney using SHANEL histology
3D reconstruction of human kidney cortex showing TRITC-dextran labeled vessels and TO-PRO-3 labeled glomeruli structures, imaged by light-sheet microscopy. Individual glomerulus was tracked by afferent arteriole. Structural profiles of cortex were characterized by the width, glomerular capillary diameter and afferent arteriole diameter.