Movie S1
Part 1) vDISCO whole-body cleared LysM mouse demonstrates the distribution of monocytes and neutrophils in the whole body after MCAo
Part 2) vDISCO cleared LysM mouse head after MCAo has high cell intensity as well as LysM+ immune cells in the parietal and frontal skull related to Figures 1 and 2

Movie S2
Part 1) Tissue cleared and light sheet imaged human skull-meninges-brain block demonstrates the channel structure,
Part 2) IBA1+ labeled human skull+meninges (dura) sample displays immune cells in the skull-meninges connections,
Part 3) LYZ2 and lectin labeling on the human skull+dura sample shows an overview of skull structure, demonstrates channels going through the dura related to Figure 4

Movie S3
LYZ2 and lectin labeling on human skull+dura sample shows an overview of skull structure and demonstrates a diploic vein and SMCs related to Figure 4

Movie S4
TSPO-PET signal from an Alzheimer’s patient shows a high TSPO-PET signal coming from the calvaria related to Figure 6